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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. S.; BARROS, B. A. F. de; MONTEIRO, C. A. S.; ROSA, P. M. S.; LEAL, G. R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; B. A. F. de Barros; C. A. S. Monteiro; P. M. S. Rosa; G. R. Leal; Raquel Varela Serapião; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Individual assessment of bovine embryo development using a homemade chamber reveals kinetic patterns of success and failure to reach blastocyst stage. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Systems Biology in Reproductive Medicine, v. 65, n. 4, p. 301-311, 2019. |
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DOI: |
https://doi.org/10.1080/19396368.2019.1589601 |
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Idioma: |
Inglês |
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Conteúdo: |
Most early developmental data are lost in bovine embryo culture systems. We developed and validated a method for culture of bovine embryos in groups that allow individual assessment. An autoclavable low-cost multiembryo chamber (MEC) was prepared using a polyester mesh fixed to a glass coverslip. Embryonic development was not affected by MEC. Compared to conventional bovine culture system (oil-covered drops, control), cleavage (C, 71.2 ± 7.8%; MEC, 74.3 ± 6.0%), blastocyst rate (C, 29.9 ± 4.4%; MEC, 28.3 ± 5.0%) and blastocyst cell number (C, 94.1 ± 9.7; MEC, 92.9 ± 5.3) were similar. Caspase 3 positive cell index in blastocysts was increased in MEC group, but apoptosis rate was below 5% (C, 2.9 ± 0.5; MEC, 4.6 ± 0.6). Using MEC, we performed a retrospective analysis for 'failure' and 'success' embryos, based on their ability to reach the blastocyst stage. We detected the majority of 'success' embryos displayed 8 cells at 48 h post-insemination (hpi) (48.7%), but blastocysts derived from this pattern presented lower cell numbers (91.3 ± 4.2 vs. 107.9 ± 4.9) and higher apoptosis index (6.2 ± 0.6 vs. 4.4 ± 0.5) than blastocysts from 4-cell embryos at 48 hpi. Most (72.0%) embryos that were at morula stage 120 hpi reached blastocyst stage at 168 hpi. Those blastocysts presented more number of cells than blastocysts derived from embryos exhibiting 16 cells at 120 hpi (108.6 ± 4.1 vs. 83.9 ± 4.8). Combination of embryo kinetics data at 48 and 120 hpi revealed high chances of blastocyst formation for patterns: 8 cells/morula, 4 cells/morula, 8 cells/16 cells and 4 cells/16 cells. Blastocysts formed from 4-cell/morula and 8-cell/morula patterns represented 69% of all 168 hpi blastocysts. Blastocysts derived from 4 cells/16 cells displayed decreased apoptosis (3.1 ± 0.6). Our results suggest that MEC can be used for bovine embryo culture without detrimental effects on development and can help to predict blastocyst formation and quality of in vitro fertilization (IVF) embryos. Abbreviations: BSA: bovine serum albumine; COC: cumulus-oocyte complex; FERT-TALP: Tyrode's albumin lactate pyruvate fertilization; FBS: fetal bovine serum; IVF: in vitro fertilization; MEC: multiembryo chamber; PBS: phosphate buffered saline; SOF-AA: synthetic oviductal fluid with amino acids medium; TCM: Tissue Culture Medium. MenosMost early developmental data are lost in bovine embryo culture systems. We developed and validated a method for culture of bovine embryos in groups that allow individual assessment. An autoclavable low-cost multiembryo chamber (MEC) was prepared using a polyester mesh fixed to a glass coverslip. Embryonic development was not affected by MEC. Compared to conventional bovine culture system (oil-covered drops, control), cleavage (C, 71.2 ± 7.8%; MEC, 74.3 ± 6.0%), blastocyst rate (C, 29.9 ± 4.4%; MEC, 28.3 ± 5.0%) and blastocyst cell number (C, 94.1 ± 9.7; MEC, 92.9 ± 5.3) were similar. Caspase 3 positive cell index in blastocysts was increased in MEC group, but apoptosis rate was below 5% (C, 2.9 ± 0.5; MEC, 4.6 ± 0.6). Using MEC, we performed a retrospective analysis for 'failure' and 'success' embryos, based on their ability to reach the blastocyst stage. We detected the majority of 'success' embryos displayed 8 cells at 48 h post-insemination (hpi) (48.7%), but blastocysts derived from this pattern presented lower cell numbers (91.3 ± 4.2 vs. 107.9 ± 4.9) and higher apoptosis index (6.2 ± 0.6 vs. 4.4 ± 0.5) than blastocysts from 4-cell embryos at 48 hpi. Most (72.0%) embryos that were at morula stage 120 hpi reached blastocyst stage at 168 hpi. Those blastocysts presented more number of cells than blastocysts derived from embryos exhibiting 16 cells at 120 hpi (108.6 ± 4.1 vs. 83.9 ± 4.8). Combination of embryo kinetics data at 48 and 120 hpi revealed high chances of blast... Mostrar Tudo |
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Palavras-Chave: |
Culture system; Embryo kinetics; Embryonic quality; Single embryo culture. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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024 7 $ahttps://doi.org/10.1080/19396368.2019.1589601$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aIndividual assessment of bovine embryo development using a homemade chamber reveals kinetic patterns of success and failure to reach blastocyst stage.$h[electronic resource]
260 $c2019
520 $aMost early developmental data are lost in bovine embryo culture systems. We developed and validated a method for culture of bovine embryos in groups that allow individual assessment. An autoclavable low-cost multiembryo chamber (MEC) was prepared using a polyester mesh fixed to a glass coverslip. Embryonic development was not affected by MEC. Compared to conventional bovine culture system (oil-covered drops, control), cleavage (C, 71.2 ± 7.8%; MEC, 74.3 ± 6.0%), blastocyst rate (C, 29.9 ± 4.4%; MEC, 28.3 ± 5.0%) and blastocyst cell number (C, 94.1 ± 9.7; MEC, 92.9 ± 5.3) were similar. Caspase 3 positive cell index in blastocysts was increased in MEC group, but apoptosis rate was below 5% (C, 2.9 ± 0.5; MEC, 4.6 ± 0.6). Using MEC, we performed a retrospective analysis for 'failure' and 'success' embryos, based on their ability to reach the blastocyst stage. We detected the majority of 'success' embryos displayed 8 cells at 48 h post-insemination (hpi) (48.7%), but blastocysts derived from this pattern presented lower cell numbers (91.3 ± 4.2 vs. 107.9 ± 4.9) and higher apoptosis index (6.2 ± 0.6 vs. 4.4 ± 0.5) than blastocysts from 4-cell embryos at 48 hpi. Most (72.0%) embryos that were at morula stage 120 hpi reached blastocyst stage at 168 hpi. Those blastocysts presented more number of cells than blastocysts derived from embryos exhibiting 16 cells at 120 hpi (108.6 ± 4.1 vs. 83.9 ± 4.8). Combination of embryo kinetics data at 48 and 120 hpi revealed high chances of blastocyst formation for patterns: 8 cells/morula, 4 cells/morula, 8 cells/16 cells and 4 cells/16 cells. Blastocysts formed from 4-cell/morula and 8-cell/morula patterns represented 69% of all 168 hpi blastocysts. Blastocysts derived from 4 cells/16 cells displayed decreased apoptosis (3.1 ± 0.6). Our results suggest that MEC can be used for bovine embryo culture without detrimental effects on development and can help to predict blastocyst formation and quality of in vitro fertilization (IVF) embryos. Abbreviations: BSA: bovine serum albumine; COC: cumulus-oocyte complex; FERT-TALP: Tyrode's albumin lactate pyruvate fertilization; FBS: fetal bovine serum; IVF: in vitro fertilization; MEC: multiembryo chamber; PBS: phosphate buffered saline; SOF-AA: synthetic oviductal fluid with amino acids medium; TCM: Tissue Culture Medium.
653 $aCulture system
653 $aEmbryo kinetics
653 $aEmbryonic quality
653 $aSingle embryo culture
700 1 $aBARROS, B. A. F. de
700 1 $aMONTEIRO, C. A. S.
700 1 $aROSA, P. M. S.
700 1 $aLEAL, G. R.
700 1 $aSERAPIÃO, R. V.
700 1 $aCAMARGO, L. S. de A.
773 $tSystems Biology in Reproductive Medicine$gv. 65, n. 4, p. 301-311, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 26 | |
| 4. |  | NEGRI, B. F.; AZEVEDO, G. C.; LANA, U. G. P.; BARROS, B. A.; GUIMARAES, C. T.; SOUSA, S. M. de. Detailed expression analysis of maize Pstol1 homologs in contrasting genotypes for phosphorus efficiency. In: ANNUAL MAIZE GENETICS CONFERENCE, 57., 2015. St. Charles, Illinois. Program and abstracts... [S.l: s.n.], 2015. p. 91.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 5. | | MACIEL, L. S.; BARROS, B. A.; DAMASCENO, C. M. B.; SOUSA, S. M. de; GUIMARAES, C. T.; MAGALHAES, J. V. Functional characterization of Phosphorus Starvation Tolerance1 genes in sorghum (SbPSTOL1). In: CONGRESSO BRASILEIRO DE GENÉTICA, 62., 2016, Caxambu. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | NEGRI, B. F.; AZEVEDO, G. C.; LANA, U. G. P.; BARROS, B. A.; GUIMARAES, C. T.; SOUSA, S. M. de. Spatial and temporal gene expression of ZmPSTOL1 genes in maize. In: CONGRESSO BRASILEIRO DE GENÉTICA, 60., 2014, Guarujá. Resumos. Ribeirão Preto: Sociedade Brasileira de Genética, 2014. p. 69.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 8. | | VIEIRA, S.; OLIVEIRA, L. G.; MAURICIO, R. M.; SOUZA, M. R.; BARROS, B. A.; MARRIEL, I. E.; SILVA, A. M. Isolamento e identificação de bactérias ácido lácticas de solo e pastagens de fazendas produtoras de queijo minas artesanal. In: CONGRESSO LATINOAMERICANO DE MICROBIOLOGIA, 21., 2012, Santos. Resumos... Santos: Sociedade Brasileira de Microbiologia, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | LIVIO, D. F.; DINIZ, M. N.; BARROS, B. A.; SOUZA, V. F. de; PASTINA, M. M.; NODA, R. W.; DAMASCENO, C. M. B. Allelic variation in HCT1 gene expression is associated with lignin content in sorghum. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 6., 2017, Ouro Preto. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 10. | | GONÇALVES, I. M.; LANA, U. G. P.; SOUZA, I. R. P.; CARVALHO, S. G. M.; NODA, R. W.; GUIMARAES, C. T.; MELO, N. D.; BARROS, B. A. Resistance gene analogs and STS markers colocalized with a major QTL conferring resistance to SCMV in tropical maize. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 221.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 11. | | DAMASCENO, C. M. B.; SOUZA, V. F.; BARROS, B. A.; SIMEONE, M. L. F.; PASTINA, M. M.; MAGALHAES, J. V.; PARRELLA, R. A. C.; SCHAFFERT, R. E. Correlation of lignin gene expression and biomass quality for biofuel production in sorghum. In: CONGRESSO BRASILEIRO DE GENÉTICA, 62., 2016, Caxambu. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 12. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASSOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Caracterização de oócitos de animais pré-púberes da raga Gir: tamnho do oócito, morfologia e atividade metabólica. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | BARROS, B. A.; KUSTER, L. M.; SILVA, L. F.; LANA, U. G. P.; MAGALHAES, J. V.; CARNEIRO, A. A.; ALVES, V. M. C.; GUIMARAES, C. T. Overexpression of SbMATE gene improves aluminum tolerance in maize. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 268.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 14. | | RAFAEL, H. A.; SOUZA, I. R. P. de; BARROS, B. A.; PINTO, M. O.; MOREIRA, R. O.; GONÇALVES, I. A. M.; CARVALHO, S. G. M. Molecular detection of the Sugarcane Mosaic Virus causing mosaic disease in maize and sorghum in Brazil. In: CONGRESSO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Ribeirão Preto: SBG, 2013. p. 94.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 15. | | MONTEIRO, C. A. S.; BARROS, B. A. F. De; ROSA, P. A. Da S.; LEAL, G. R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Taxa de apoptose após vitrificação/ reaquecimento de embriões Gir produzidos in vitro. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. 1| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | ROSA, P. M. Da S.; BARROS, B. A. F. De; PASOLINI, R.; CAMARGO, A. J. Dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Hormonal ovulation induction in prepubertal GYR heifers In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tours: Society for Reproductive Biology, 2016. p. 516-517.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | OLIVEIRA, C. S.; BARROS, B. A. F. de; MONTEIRO, C. A. S.; ROSA, P. M. S.; LEAL, G. R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A. Individual assessment of bovine embryo development using a homemade chamber reveals kinetic patterns of success and failure to reach blastocyst stage. Systems Biology in Reproductive Medicine, v. 65, n. 4, p. 301-311, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | ROSA, P. M. da S.; BARROS, B. A. F. de; PASOLINI, R.; CAMARGO, A. J. dos R.; SERAPIÃO, R. V.; OLIVEIRA, C. S. Indução de ovulação em novilhas pré-púberes da raça Gir. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 17., 2016, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2016. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 186).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | SOUZA, I. R. P. de; CARVALHO, S. G. M.; MELO, N. O.; GONÇALVES, I. A. M.; SABATO, E. O.; RODRIGUES, J. A. S.; BARROS, B. A. Identification potyviruses species causing mosaic symptoms in Poaceae-weed species found in maize crop. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls. Abstracts. [S.l.]: International Society for Plant Molecular Biology, 2015. p. 92.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 20. | | PINTO, M. O.; SOUZA, I. R. P.; CARVALHO, S. G. M.; MACHADO, L. M.; RAFAEL, H. A.; GONÇALVES, I. A. M.; BARROS, B. A.; CARNEIRO, N. P. Marker-assisted selection for genome recovering and introgression of resistance to maize common mosaic. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 4., 2013, Bento Gonçalves. Resumos... Bento Gonçalves: Sociedade Brasileira de Genética, 2013. p. 47.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 26 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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